Publications — BoeynaemsLab

Publication List

Preprints

Field S, Dorone Y, Dwyer WP, Cox JA, Raba D, Froehlich J, Blea M, Carmo OMS, Wallace IS, Hastings R, Boeynaems S, Rhee SY. Arabidopsis thaliana RHAMNOSE 1 condensate formation drives UDP-rhamnose synthesis. BioRxiv 2024.

Boeynaems S#, Ma XR, Yeong V, Ginell GM, Chen JH, Blum JA, Nakayama L, Sanyal A, Briner A, Van Haver D, Pauwels J, Ekman A, Schmidt HB, Sundararajan K, Porta L, Lasker K, Larabell C, Hayashi MAF, Kundaje A, Impens F, Obermeyer A, Holehouse AS, Gitler AD#. Aberrant phase separation is a common killing strategy of positively charged peptides in biology and human disease. BioRxiv 2023. co-corresponding

2023

Xu WQ, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor JP, Udeshi ND, Carr SA, Ting AY. Dynamic mapping of proteome trafficking within and between living cells by TransitID. Cell 2023.

Boeynaems S#, Dorone Y, Zhuang Y, Shabardina V, Huang G, Anca M, Kim G, Sanyal A, Sen N-E, Griffith D, Docampo R, Lasker K, Ruiz-Trillo I, Auburger G, Holehouse AS, Kabashi E, Lin Y, Gitler AD#. Poly(A)-binding protein is an ataxin-2 chaperone that regulates biomolecular condensates. Molecular Cell 2023. co-corresponding

Preview in Molecular Cell

Romero-Perez PS*, Dorone Y*, Flores E, Sukenik S#, Boeynaems S#. When phased without water: biophysics of cellular desiccation, from biomolecules to condensates. Chemical Reviews 2023. co-corresponding

Onuchic P & Boeynaems S. You're in then you're out. Nature Chemical Biology 2023, vol. 19(4), 399-400.

Boeynaems S, Chong S, Gsponer J, Holt L, Milovanovic D, Mitrea DM, Mueller-Cajar O, Portz B, Reilly JF, Reinkemeier CD, Sabari BR, Sanulli S, Shorter J, Sontag E, Strader L, Stachowiak J, Weber SC, White M, Zhang H, Zweckstetter M, Elbaum-Garfinkle S, Kriwacki R. Phase Separation in Biology and Disease; Current Perspectives and Open Questions. Journal of Molecular Biology 2023, vol. 435(5), 167971. all authors contributed equally

2022

Kim G, Nakayama L, Blum JA, Akiyama T, Boeynaems S, Chakraborty M, Couthouis J, Tassoni-Tsuchida E, Rodriguez CM, Bassik MC, Gitler AD. Genome-wide CRISPR screen reveals v-ATPase as a drug target to lower levels of ALS protein ataxin-2. Cell Reports 2022, vol. 41(4), 111508.

Lasker K*, Boeynaems S*, Lam V, Scholl D, Stainton E, Briner A, Jacquemyn M, Daelemans D, Deniz A, Villa E, Holehouse AS, Gitler AD, Shapiro L. The material properties of a bacterial-derived biomolecular condensate tune biological function in natural and synthetic systems. Nature Communications 2022, vol. 13, 5643. co-first

Vanneste J, Vercruysse T, Boeynaems S, Van Damme P, Daelemans D, Van Den Bosch L. Cellular stress induces nucleocytoplasmic transport deficits independent of stress granules. Biomedicines 2022, vol. 10(5), 1057.

Boeynaems S# and Gitler AD#. You come at the misfolded proteins, you best not miss. Trends in Biochemical Sciences 2022, vol. 47(1), p. 1-2. co-corresponding

2021

Dorone Y, Boeynaems S#, Rhee SY#. Imaging Arabidopsis thaliana embryos under different hydration conditions. STAR protocols 2021, vol. 2(4).

Dorone Y*, Boeynaems S*, Jin B, Bossi F, Flores E, Lazarus E, Michiels E, De Decker M, Baatsen P, Holehouse AS, Sukenik S, Gitler AD, Rhee SY. A prion-like protein regulator of seed germination undergoes hydration-dependent phase separation. Cell 2021, vol. 184(16), p. 4284-4298. co-first
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Gabarre S, Vernaillen F, Baatsen P, Vints K Cawthorne C, Boeynaems S, Michiels E, Vandael D, Gunko N, Munck S. A workflow for streamlined acquisition and correlation of serial regions of interest in array tomography. BMC Biology 2021, vol. 19, DOI: 10.1186/s12915-021-01072-7.

Friedman AK, Boeynaems S#, Baker LA#. Synthetic hydrogel mimics of the nuclear pore complex for the study of nucleocytoplasmic transport defects in C9orf72 ALS/FTD. Analytical and Bioanalytical Chemistry 2021, DOI: 10.1007/s00216-021-03478-2. co-corresponding

Fumagalli F*, Young FL*, Boeynaems S*, De Decker M, Mehta A, Swijsen A, Fazal R, Guo W, Moisse M, Beckers J, Dedeene L, Bhuvaneish TS, Vandoorne T, Madan V, van Blitterswijk M, Raitcheva D, McCampbell A, Poesen K, Gitler AD, Koch P, Vanden Berghe P, Thal DR, Verfaillie C, Chandran S, Van Den Bosch L, Bullock S, Van Damme P. C9orf72-derived arginine-containing dipeptide repeats associate with axonal transport machinery and impede microtubule-based motility. Science Advances 2021, vol. 7(15), eabg3013. co-first

Fazal R, Boeynaems S, Swijsen A, De Decker M, Fumagalli L, Moisse M, Vanneste J, Guo W, Boon R, Vercruysse T, Eggermont K, Swinnen B, Beckers J, Pakravan D, Vandoorne T, Vanden Berghe P, Verfaillie CM, Van Damme P. HDAC6 inhibition restores TDP-43 pathology and axonal transport defects in human motor neurons with TARDBP mutations. The EMBO Journal, vol. 40, e106177.

Hasting RL and Boeynaems S#. Designer condensates: a toolkit for the biomolecular architect. Journal of Molecular Biology 2021, vol. 433(12), 166837. corresponding
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2020

Tazelaar GHP*, Boeynaems S*, De Decker M*, van Vugt JJFA, Kool L, Goedee HS, McLaughlin RL, Sproviero W, Iacoangeli A, Moisse M, Jacquemyn M, Daelemans D, Dekker AM, van der Spek RA, Westeneng HJ, Kenna KP, Assialioui A, Da Silva N, Project MinE ALS Sequencing Consortium, Povedano M, Mora Pardina JS, Hardiman O, Salachas F, Millecamps S, Vourc’h P, Corcia P, Couratier P, Morrison KE, Shaw PJ, Shaw CE, Pasterkamp RJ, Landers JE, Van Den Bosch L, Robberecht W, Al-Chalabi A, van den Berg LH, Van Damme P, Veldink JH, van Es MA. ATXN1 repeat expansions confer risk for amyotrophic lateral sclerosis and contribute to TDP-43 mislocalization. Brain Communications, fcaa064. co-first

Gittings L, Boeynaems S, Lightwood D, Clargo A, Topia S, Nakayama L, Troakes C, Mann D, Gitler AD, Lashley T, Isaacs A. Symmetric dimethylation of poly-GR correlates with disease duration in C9orf72 FTLD and ALS and reduces poly-GR phase separation and toxicity. Acta Neuropathologica, vol. 139, p. 407–410.

2019

Vanneste J, Vercruysse T, Boeynaems S, Sicart A, Van Damme P, Daelemans D, Van Den Bosch L. C9orf72-generated poly-GR and poly-PR do not directly interfere with nucleocytoplasmic transport. Scientific Reports, vol. 9, article number: 15728.

Boeynaems S and Gitler AD. Axons Gonna Ride 'til They Can't No More. Neuron, vol. 104(2), p. 179-181.

Boeynaems S, Holehouse AS, Weinhardt V, Kovacs D, Van Lindt J, Larabell C, Van Den Bosch L, Das R, Tompa P, Pappu RV, Gitler AD. Spontaneous driving forces give rise to protein-RNA condensates with coexisting phases and complex material properties. PNAS, vol. 116(16), p. 7889-7898. co-corresponding

2018

Boeynaems S and Gitler AD. Pour Some Sugar on TDP (-43). Molecular Cell, vol. 71(5), p. 649-651.

Bogaert E*, Boeynaems S*, Kato M, Guo L, Caulfield TR, Steyaert S, Scheveneels W, Wilmans N, Haeck W, Hersmus N, Schymkowitz S, Rousseau F, Shorter J, Callaerts P, Robberecht W, Van Damme P, Van Den Bosch L. Molecular dissection of FUS points at synergistic effect of low-complexity domains in toxicity. Cell Reports, vol. 24(3), p. 529-537. co-first, co-corresponding
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Boeynaems S, Alberti S, Fawzi NL, Mittag T, Polymenidou M, Rousseau F, Schymkowitz J, Shorter J, Wolozin B, Van Den Bosch L, Tompa T, Fuxreiter M. Protein phase separation: a new phase in cell biology. Trends in Cell Biology, vol. 28(6), p. 420–435. co-corresponding
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Boeynaems S and Gitler AD. Phosphorylation Leads the Way for Protein Aggregate Disassembly. Developmental Cell , vol. 45(3), p. 279-281.

Swinnen B, Boeynaems S, Schrooten M, Saegeman V, Claeys KG, Van Damme P. Anterior interosseous mononeuropathy associated with HEV infection. Neurology - Neuroimmunology Neuroinflammation, vol. 5(2), e429.

Swinnen B, Bento-Abreu A, Gendron TF, Boeynaems S, Bogaert E, Nuyts R, Timmers M, Scheveneels W, Hersmus N, Wang J, Mizielinska S, Isaacs A, Petrucelli L, Van Damme P, Van Den Bosch L, Robberecht W. A zebrafish model for C9orf72 ALS reveals RNA toxicity as a pathogenic mechanism. Acta Neuropathologica, vol. 135(3), p. 427–443.

Boeynaems S, Tompa P, Van Den Bosch L. Phasing in on the cell cycle. Cell Division, vol. 13(1). corresponding

2017

Boeynaems S, De Decker M, Tompa P, Van Den Bosch L. Arginine-rich Peptides Can Actively Mediate Liquid-liquid Phase Separation. Bio-protocol, vol. 7(17).

Boeynaems S, Bogaert E, Kovacs D, Konijnenberg A, Timmerman E, Volkov A, Guharoy M, De Decker M, Jaspers T, Ryan VH, Janke AM, Baatsen P, Vercruysse T, Kolaitis RM, Daelemans D, Taylor JP, Kedersha N, Anderson P, Impens F, Sobott F, Schymkowitz J, Rousseau F, Fawzi NL, Robberecht W, Van Damme P, Tompa P, Van Den Bosch L. Phase Separation of C9orf72 Dipeptide Repeats Perturbs Stress Granule Dynamics. Molecular Cell, vol. 65(6), p. 1044-1055.
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2016

Boeynaems S, Bogaert E, Van Damme P, Van Den Bosch L. Inside out: the role of nucleocytoplasmic transport in ALS and FTLD. Acta Neuropathologica, vol. 132(2), p. 159-173. co-corresponding

Boeynaems S*, Bogaert E*, Michiels E, Gijselinck I, Sieben A, Jovicic A, De Baets G, Scheveneels W, Steyaert J, Cuijt I, Verstrepen KJ, Callaerts P, Rousseau F, Schymkowitz J, Cruts M, Van Broeckhoven C, Van Damme P, Gitler AD, Robberecht W, Van Den Bosch L. Drosophila screen connects nuclear transport genes to DPR pathology in c9ALS/FTD. Scientific Reports, vol. 6, article number: 20877. co-first

2015

Jovicic A, Mertens J, Boeynaems S, Bogaert E, Chai N, Yamada SB, Paul JW 3rd, Sun S, Herdy JR, Bieri G, Kramer NJ, Gage FH, Van Den Bosch L, Robberecht W, Gitler AD. Modifiers of C9orf72 dipeptide repeat toxicity connect nucleocytoplasmic transport defects to FTD/ALS. Nature Neuroscience, vol. 18(9), p. 1226-1229.

Gemayel R, Chavali S, Pougach K, Legendre M, Zhu B, Boeynaems S, van der Zande E, Gevaert K, Rousseau F, Schymkowitz J, Babu MM, Verstrepen KJ. Variable glutamine-rich repeats modulate transcription factor activity. Molecular Cell, vol. 59(4), p. 615-627.